, A was purified by CC with different mixtures of hexane/EtOAc yielding 157 fractions. The ones (36-40) eluted in the ratio 9:1 yielded lupeol (29.5 mg). From the fraction B, 90 fractions were obtained and stigmasterol (240 mg) was isolated from the fractions 30-40 eluted in the ratio 17:3. From the same fraction B, 5 mg of betulinic acid were isolated from the fractions 42-44 eluted with the mixture Hexane/EtOAc (4:1). EtOAc fraction (D) was further eluted with a mixture of CH 2 Cl 2 /MeOH with increasing polarity. 104 fractions was obtained and from the ones 20-36 eluted with the mixture CH 2 Cl 2 /MeOH (19:1), the 3-O-?-D-glucopyranoside of ?-sitosterol (11.0 mg) was isolated, EtOAc in order of increasing polarity and MeOH) yielding 5 fractions A-E

, Dark amorphous solid

, FT-IR: 3368, 1649, 1637, 1542, and 1468. 1 H-and 13 C-NMR: Table 1

. Esi-ms-ms, , p.624

, H 97 O 11 N -CH 3 OH-C 18 H 36 +Na] +

. Fab-ms,

J. Berhaut and . Flore, , vol.VI, p.471, 1979.

E. S. Abdel-hameed, Food Chemistry, vol.114, p.1271, 2009.

M. S. Diehl, K. K. Atindehou, H. Téré, and B. Betschart, J. Ethnopharmacol, p.277, 2004.

N. K. Ayissi and C. Nyadedzor, Antiviral Research, vol.58, p.25, 2003.

V. Kuete, B. Ngameni, C. C. Simo, R. K. Tankeu, B. T. Ngadjui et al., J. Ethnopharmacol, vol.120, p.17, 2008.

E. D. Walter, J. Pharm. Sci, vol.52, p.708, 1963.

S. Siddiqui, F. Hafeez, S. Begum, and B. S. Siddiqui, J. Nat. Prod, p.229, 1988.

W. De-eknamkul and B. Potduang, Phytochemistry, vol.62, p.389, 2003.

W. F. Reynolds, S. Mclean, J. Poplawski, R. G. Enriquez, L. I. Escobar et al., Tetrahedron, p.3419, 1986.

S. Y. Kim, Y. Choi, H. Huh, J. Kim, Y. C. Kim et al., J. Nat. Prod, vol.60, p.274, 1997.

L. P. Sandjo, P. Hannewald, M. Yemloul, G. Kirsch, and B. T. Ngadjui, Helv. Chim. Acta, p.1326, 2008.

Y. Chen, C. Tan, J. Tan, S. Qu, H. Wang et al., Helv. Chim. Acta, p.2421, 2007.