Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila - Université de Versailles Saint-Quentin-en-Yvelines Access content directly
Journal Articles Microbiology Spectrum Year : 2023

Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila

Abstract

Mycobacterium abscessus, an intracellular nontuberculous mycobacterium, is considered the most pathogenic species among the group of rapidly growing mycobacteria. The resistance of M. abscessus to the host innate response contributes to its pathogenicity in addition to several virulence factors. We have recently shown in Drosophila that anti-microbial peptides (AMPs), whose production is induced by M. abscessus, are unable to control mycobacterial infection. This could be due to their inability to kill mycobacteria and/or the hidden location of the pathogen in phagocytic cells. Here, we demonstrate that the rapid internalization of M. abscessus by Drosophila macrophages allows it to escape the AMP-mediated humoral response. By depleting phagocytes in AMP-deficient flies, we found that several AMPs were required for the control of extracellular M. abscessus. This was confirmed in the Tep4 opsonin-deficient flies, which we show can better control M. abscessus growth and have increased survival through overproduction of some AMPs, including Defensin. Furthermore, Defensin alone was sufficient to kill extracellular M. abscessus both in vitro and in vivo and control its infection. Collectively, our data support that Tep4-mediated opsonization of M. abscessus allows its escape and resistance toward the Defensin bactericidal action in Drosophila
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Dates and versions

hal-04114156 , version 1 (01-06-2023)
hal-04114156 , version 2 (19-08-2023)

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Hamadoun Touré, Nicolas Durand, Isabelle Guénal, Jean-Louis Herrmann, Fabienne Girard-Misguich, et al.. Mycobacterium abscessus Opsonization Allows an Escape from the Defensin Bactericidal Action in Drosophila. Microbiology Spectrum, 2023, 11 (4), pp.e0077723. ⟨10.1128/spectrum.00777-23⟩. ⟨hal-04114156v2⟩
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