The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
Mirta Milić
(1)
,
Marcello Ceppi
(2)
,
Marco Bruzzone
(2)
,
Amaya Azqueta
(3, 4)
,
Gunnar Brunborg
(5)
,
Roger Godschalk
(6)
,
Gudrun Koppen
(7)
,
Sabine a S Langie
(6)
,
Peter Møller
(8)
,
João Paulo Teixeira
(9, 2, 8)
,
Avdulla Alija
(10)
,
Diana Anderson
(11)
,
Vanessa Andrade
(12)
,
Cristina Andreoli
(13)
,
Fisnik Asllani
(10)
,
Ezgi Eyluel Bangkoglu
(14)
,
Magdalena Barančoková
(15)
,
Nursen Basaran
(16)
,
Elisa Boutet-Robinet
(17)
,
Annamaria Buschini
(18)
,
Delia Cavallo
(19)
,
Cristiana Costa Pereira
(9, 2, 8)
,
Carla Costa
(9, 2, 8)
,
Solange Costa
(9, 2, 8)
,
Juliana da Silva
(20)
,
Cristian del Boˊ
(21)
,
Vesna Dimitrijević Srećković
(22)
,
Ninoslav Djelić
(23)
,
Malgorzata Dobrzyńska
(24)
,
Zdenka Duračková
(25)
,
Monika Dvořáková
(25)
,
Goran Gajski
(1)
,
Serena Galati
(26)
,
Omar García Lima
(27)
,
Lisa Giovannelli
(28)
,
Irina Goroshinskaya
(29)
,
Annemarie Grindel
(30)
,
Kristine Gutzkow
(5)
,
Alba Hernández
(31, 32)
,
Carlos Hernández
(33)
,
Kirsten Holven
(34)
,
Idoia Ibero-Baraibar
(3)
,
Inger Ottestad
(34)
,
Ela Kadioglu
(35)
,
Alena Kažimirová
(15)
,
Elena Kuznetsova
(36)
,
Carina Ladeira
(37, 38)
,
Blanca Laffon
(39)
,
Palma Lamonaca
(40)
,
Pierre Lebailly
(41)
,
Henriqueta Louro
(38)
,
Tania Mandina Cardoso
(27)
,
Francesca Marcon
(13)
,
Ricard Marcos
(31, 32)
,
Massimo Moretti
(42)
,
Silvia Moretti
(43)
,
Mojgan Najafzadeh
(8)
,
Zsuzsanna Nemeth
(44)
,
Monica Neri
(45)
,
Bozena Novotna
(46)
,
Irene Orlow
(47)
,
Zuzana Paduchova
(25)
,
Susana Pastor
(31, 32)
,
Hervé Perdry
(48)
,
Biljana Spremo-Potparević
(49)
,
Dwi Ramadhani
(50)
,
Patrizia Riso
(21)
,
Paula Rohr
(12)
,
Emilio Rojas
(51)
,
Pavel Rossner
(46)
,
Anna Safar
(44)
,
Semra Sardas
(52)
,
Maria João Silva
(38)
,
Nikolay Sirota
(53)
,
Bozena Smolkova
(54)
,
Marta Staruchova
(15)
,
Rudolf Stetina
(55)
,
Helga Stopper
(14)
,
Ekaterina Surikova
(29)
,
Stine Ulven
(34)
,
Cinzia Lucia Ursini
(19)
,
Vanessa Valdiglesias
(56)
,
Mahara Valverde
(51)
,
Pavel Vodicka
(57)
,
Katarina Volkovova
(15)
,
Karl-Heinz Wagner
(30)
,
Lada Živković
(58)
,
Maria Dušinská
(59)
,
Andrew Collins
(34)
,
Stefano Bonassi
(45)
1
Institute for Medical Research and Occupational Health
2 Ospedale Policlinico San Martino [Genoa]
3 UNAV - Universidad de Navarra [Pamplona]
4 CIMA - Center for Applied Medical Research [Plamplona]
5 NIPH - Norwegian Institute of Public Health [Oslo]
6 NUTRIM - School of Nutrition and Translational Research in Metabolism [Maastricht]
7 VITO - Flemish Institute for Technological Research
8 UCPH - University of Copenhagen = Københavns Universitet
9 INSA - Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal]
10 University of Prishtina
11 University of Bradford
12 UNISUL - University of Southern Santa Catarina [Florianopolis]
13 ISS - Istituto Superiore di Sanità = National Institute of Health
14 University of Würzburg
15 SMU - Slovak Medical University of Bratislava
16 Hacettepe University = Hacettepe Üniversitesi
17 ToxAlim-COMICS - Contaminants & Stress Cellulaire
18 Department of Chemistry, Life Sciences and Environmental Sustainability [Parma]
19 INAIL - Istituto Nazionale per l’Assicurazione contro gli Infortuni sul Lavoro [Italian Workers Compensation Authority]
20 Universidade Luterana do Brasil - ULBRA (BRAZIL)
21 Department of Food Environmental and Nutritional Sciences, (DeFENS)
22 School of Medecine [Belgrade]
23 Faculty of Veterinary Medicine [Belgrade, Serbie]
24 National Institute of Public Health - National Institute of Hygiene [Poland]
25 Comenius University in Bratislava
26 UNIPR - Università degli studi di Parma = University of Parma
27 CPHR - Centro de Protección e Higiene de las Radiaciones [Cuba]
28 NEUROFARBA Department [Firenze, Italy]
29 Petrov Research Insitut of Oncology
30 University of Vienna [Vienna]
31 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
32 Institute of Health Carlos III
33 Instituto de Ciencias Básicas y Preclínicas Victoria de Girón
34 Institute of Basic Medical Sciences [Oslo]
35 Gazi University
36 Institute Of Theoretical And Experimental Biophysics
37 Escola Superior de Tecnologia da Saude de Lisboa
38 NOVA - Universidade Nova de Lisboa = NOVA University Lisbon
39 UDC - University of A Coruña
40 IRCCS - Istituto di Ricovero e Cura a Carattere Scientifico, Ospedale Casa Sollievo della Sofferenza [San Giovanni Rotondo]
41 ANTICIPE - Unité de recherche interdisciplinaire pour la prévention et le traitement des cancers
42 Dept. of Pharmaceutical Sciences, University of Perugia
43 UniFI - Università degli Studi di Firenze = University of Florence = Université de Florence
44 National center for public health [Hungary]
45 IRCCS San Raffaele Pisana - Istituto di Ricovero e Cura a Carattere Scientifico
46 CAS - Czech Academy of Sciences [Prague]
47 Memorial Sloane Kettering Cancer Center [New York]
48 CESP - Centre de recherche en épidémiologie et santé des populations
49 Faculty of Pharmacy
50 BATAN - National Nuclear Energy Agency of Indonesia
51 Instituto de Investigaciones Biomedicas
52 Istinye University
53 RAS - the Russian Academy of Sciences [Moscow, Russia]
54 SAS - Slovak Academy of Science [Bratislava]
55 FMT / UoD - Faculty of Military Technology [Brno]
56 CICA - Centro de Investigacións Científicas Avanzadas
57 Institute of Clinical and Experimental Medicine
58 University of Belgrade [Belgrade]
59 NILU - Norsk Institutt for Luftforskning
2 Ospedale Policlinico San Martino [Genoa]
3 UNAV - Universidad de Navarra [Pamplona]
4 CIMA - Center for Applied Medical Research [Plamplona]
5 NIPH - Norwegian Institute of Public Health [Oslo]
6 NUTRIM - School of Nutrition and Translational Research in Metabolism [Maastricht]
7 VITO - Flemish Institute for Technological Research
8 UCPH - University of Copenhagen = Københavns Universitet
9 INSA - Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal]
10 University of Prishtina
11 University of Bradford
12 UNISUL - University of Southern Santa Catarina [Florianopolis]
13 ISS - Istituto Superiore di Sanità = National Institute of Health
14 University of Würzburg
15 SMU - Slovak Medical University of Bratislava
16 Hacettepe University = Hacettepe Üniversitesi
17 ToxAlim-COMICS - Contaminants & Stress Cellulaire
18 Department of Chemistry, Life Sciences and Environmental Sustainability [Parma]
19 INAIL - Istituto Nazionale per l’Assicurazione contro gli Infortuni sul Lavoro [Italian Workers Compensation Authority]
20 Universidade Luterana do Brasil - ULBRA (BRAZIL)
21 Department of Food Environmental and Nutritional Sciences, (DeFENS)
22 School of Medecine [Belgrade]
23 Faculty of Veterinary Medicine [Belgrade, Serbie]
24 National Institute of Public Health - National Institute of Hygiene [Poland]
25 Comenius University in Bratislava
26 UNIPR - Università degli studi di Parma = University of Parma
27 CPHR - Centro de Protección e Higiene de las Radiaciones [Cuba]
28 NEUROFARBA Department [Firenze, Italy]
29 Petrov Research Insitut of Oncology
30 University of Vienna [Vienna]
31 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
32 Institute of Health Carlos III
33 Instituto de Ciencias Básicas y Preclínicas Victoria de Girón
34 Institute of Basic Medical Sciences [Oslo]
35 Gazi University
36 Institute Of Theoretical And Experimental Biophysics
37 Escola Superior de Tecnologia da Saude de Lisboa
38 NOVA - Universidade Nova de Lisboa = NOVA University Lisbon
39 UDC - University of A Coruña
40 IRCCS - Istituto di Ricovero e Cura a Carattere Scientifico, Ospedale Casa Sollievo della Sofferenza [San Giovanni Rotondo]
41 ANTICIPE - Unité de recherche interdisciplinaire pour la prévention et le traitement des cancers
42 Dept. of Pharmaceutical Sciences, University of Perugia
43 UniFI - Università degli Studi di Firenze = University of Florence = Université de Florence
44 National center for public health [Hungary]
45 IRCCS San Raffaele Pisana - Istituto di Ricovero e Cura a Carattere Scientifico
46 CAS - Czech Academy of Sciences [Prague]
47 Memorial Sloane Kettering Cancer Center [New York]
48 CESP - Centre de recherche en épidémiologie et santé des populations
49 Faculty of Pharmacy
50 BATAN - National Nuclear Energy Agency of Indonesia
51 Instituto de Investigaciones Biomedicas
52 Istinye University
53 RAS - the Russian Academy of Sciences [Moscow, Russia]
54 SAS - Slovak Academy of Science [Bratislava]
55 FMT / UoD - Faculty of Military Technology [Brno]
56 CICA - Centro de Investigacións Científicas Avanzadas
57 Institute of Clinical and Experimental Medicine
58 University of Belgrade [Belgrade]
59 NILU - Norsk Institutt for Luftforskning
Amaya Azqueta
- Fonction : Auteur
- PersonId : 987383
Elisa Boutet-Robinet
- Fonction : Auteur
- PersonId : 746889
- IdHAL : elisa-boutet-robinet
- ORCID : 0000-0002-5223-4568
- IdRef : 071622950
Kristine Gutzkow
- Fonction : Auteur
- PersonId : 806114
- ORCID : 0000-0002-6716-5921
Pierre Lebailly
- Fonction : Auteur
- PersonId : 1325233
- IdRef : 076947998
Patrizia Riso
- Fonction : Auteur
- PersonId : 801855
- ORCID : 0000-0002-9204-7257
Résumé
The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
Domaines
Sciences du Vivant [q-bio]
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