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Apoptosis is antagonized by large T antigens in the pathway to immortalization by polyomaviruses

Abstract : The viability of rat embryo cells immortalized by thermosensitive mutants of SV40 or polyoma Large T antigen is impaired at the non-permissive temperature thus demonstrating that the immortal phenotype is dominantly maintained by Large T antigens. We have observed that exposing these cells to the restrictive temperature not only induces growth arrest but also causes apoptotic cell death. We present evidence supporting the model that polyomaviruses may indeed establish immortality by antagonizing the lethal effects of tumor suppressor genes via physical interactions between their products and Large T antigens. In the case of SV40-immortalized cells REtsAF, shift-up to 39.5 degrees C dissociates Large T antigen/p53 complexes releasing wild-type p53 molecules capable of inducing apoptotic cell death. In polyomavirus-immortalized cells, apoptosis may result from an alternative pathway mediated by other unidentified negatively acting molecules.
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Contributor : Bernard Mignotte Connect in order to contact the contributor
Submitted on : Friday, December 18, 2020 - 3:29:36 PM
Last modification on : Thursday, April 7, 2022 - 10:10:43 AM


  • HAL Id : hal-03038441, version 1
  • PUBMED : 7936660



D.Q. Zheng, J.L. Vayssiere, P.X. Petit, Hervé Lecoeur, Alain Spatz, et al.. Apoptosis is antagonized by large T antigens in the pathway to immortalization by polyomaviruses. Oncogene, Nature Publishing Group, 1994, 9 (11), pp.3345-51. ⟨hal-03038441⟩



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