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Genetic instability from a single S phase after whole-genome duplication

Abstract : Abstract Diploid and stable karyotypes are associated with health and fitness in animals. By contrast, whole-genome duplications—doublings of the entire complement of chromosomes—are linked to genetic instability and frequently found in human cancers 1–3 . It has been established that whole-genome duplications fuel chromosome instability through abnormal mitosis 4–8 ; however, the immediate consequences of tetraploidy in the first interphase are not known. This is a key question because single whole-genome duplication events such as cytokinesis failure can promote tumorigenesis 9 and DNA double-strand breaks 10 . Here we find that human cells undergo high rates of DNA damage during DNA replication in the first S phase following induction of tetraploidy. Using DNA combing and single-cell sequencing, we show that DNA replication dynamics is perturbed, generating under- and over-replicated regions. Mechanistically, we find that these defects result from a shortage of proteins during the G1/S transition, which impairs the fidelity of DNA replication. This work shows that within a single interphase, unscheduled tetraploid cells can acquire highly abnormal karyotypes. These findings provide an explanation for the genetic instability landscape that favours tumorigenesis after tetraploidization.
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Submitted on : Thursday, November 17, 2022 - 11:27:19 AM
Last modification on : Friday, November 18, 2022 - 3:54:16 AM

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Simon Gemble, René Wardenaar, Kristina Keuper, Nishit Srivastava, Maddalena Nano, et al.. Genetic instability from a single S phase after whole-genome duplication. Nature, 2022, 604 (7904), pp.146-151. ⟨10.1038/s41586-022-04578-4⟩. ⟨hal-03857401⟩



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